Friday, March 28, 2008
Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium status of a tissue or medium. Due to the chemical properties of calcium it can not be imaged easily. Instead, another substance called Fura-2 fluoresces after binding to calcium and being exposed to a fluorescent light. The Fura-Ca complex affects the wavelength typically associated with unbound Fura-2 and the resulting fluorescence can be detected by a camera adapted (usually through a microscope) for fluorescent light imaging. An image is thus created which can be analyzed according to intensity, ultimately reflecting the Ca status.
Posted by gigihong07 at 9:17 AM